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The entire repertoire of antibodies in our serum, the IgOme, is a historical record of our past experiences and a reflection of our immune status at any given moment. Understanding the dynamics of the IgOme and how the diversity and specificities of serum antibodies change in response to disease and maintenance of homeostasis can directly impact the ability to design and develop novel vaccines, diagnostics and therapeutics.
Here we review both direct and indirect methodologies that are being developed to map the complexity and specificities of the antibodies in polyclonal serum β the IgOme.
Surprisingly, whereas pathogens come and go as they are cleared from our bodies, the antibodies generated in response to immunological insults are archived in our memory B-cells, the cells that orchestrate the continuous production of antibodies found in serum over the course of our lives. This entire utilized repertoire of antibodies in our serum, the IgOme, is a historical record of our past experiences and a reflection of our immune status at any given moment.
The answers to these questions bear directly on the development of novel vaccines, diagnostics and therapeutics and in order to meet these challenges one must be able to profile the IgOme. We need to be able to describe the IgOme in its entirety at single antibody resolution in a manner that is cost effective and expedient. One could then imagine running IgOme screens routinely in the course of personalized medical diagnosis and treatment. Obviously, the challenge is formidable β just considering the diversity of antibodies and the dynamics of their expression at any given moment and as a result to any given stimulus.
