Metrics details. The isoelectric point of 5. Therefore, the enzyme was purified from the culture supernatant and the catalytic properties and the substrate specificity of the enzyme were investigated using different assays to reveal its potential function.
The use of residual biomass for the production of daily products, food and feed is the key in the circular economy European Commission , and simultaneously partially fulfills the UN Sustainable Development Goals El-Chichakli et al.
However, the depolymerization of renewable agro-industrial residues is a challenge that can only be overcome by efficient biomass hydrolysis Ahorsu et al. A crucial step for the leap to technological applications is the production and use of efficient enzymes, which evolution has already tailored to degrade and modify biopolymers efficiently.
Especially fungi, as natural cell factories with their unique enzymes, can degrade organic lignocellulosic biomass into biogenic raw materials, which are then available as feedstocks Filiatrault-Chastel et al. Pleurotus sapidus oyster mushroom , a white-rot fungi of the phylum Basidiomycota, is an efficient lignin decomposer and able to fully degrade complex polymers Croan ; Patrick et al.
Depending on the substrate, numerous enzymes are produced by this fungus to decompose lignocellulosic biomass efficiently Zorn et al. Esterases act on ester bonds E. In nature, a great variety of different structural folds of esterases have been reported. However, especially in the group of carbohydrate esterases CEs of the carbohydrate-active enzymes CAZymes Drula et al. Typically, Gly and Asn donate protons to the oxyanion hole with Ser at the active site Upton and Buckley As a result of their wide range of applications, ester hydrolyzing enzymes are interesting tools for biotechnological processes and especially for food production Raveendran et al.
